Cell Surface Igm Or Cd23 Expression And B Cell Development In Mutant In this study we analyzed b cell development in mice deficient in both cd19 and p110δ. we found that the early stages of b cell maturation in the bone marrow were unaffected while peripheral mature b cells required the combined functions of cd19 and p110δ. Download scientific diagram | cell surface igm or cd23 expression and b cell development in mutant and wild type littermates.
Cell Surface Expression During B Cell Development The Differential Mammalian mature naïve b cells, including the b1, mzb, and fob subsets, are generated from transitional b cells that receive environmental stimuli, including signals through bcr, tlr, notch, and cytokine receptors. the lineage choice of mature b cell subsets is regulated by tfs. In the current study we use ige deficient (ige ) mice to establish the effects of ige on cd23 expression by b cells in vivo, in the absence of allergic or parasitic stimuli. the spleens of ige and wild type mice contained similar proportions of cd23 b lymphocytes. We found that baff r is expressed at higher levels on non autoreactive than autoreactive immature b cells and that its expression correlates with that of surface igm and with tonic bcr signaling. our data indicate that baff r signaling enhances the generation of transitional cd23 − b cells in vitro by increasing cell survival. Our data indicate that cd23 expression on b cells, rather than fcεri mast cells or basophils, regulates free serum ige levels. these data reveal a novel cellular mechanism for monomeric antibody clearance and suggest an additional function for circulating b cells.
Igm Surface Expression And Light Chain Usage In Cd21 Low B Cells A We found that baff r is expressed at higher levels on non autoreactive than autoreactive immature b cells and that its expression correlates with that of surface igm and with tonic bcr signaling. our data indicate that baff r signaling enhances the generation of transitional cd23 − b cells in vitro by increasing cell survival. Our data indicate that cd23 expression on b cells, rather than fcεri mast cells or basophils, regulates free serum ige levels. these data reveal a novel cellular mechanism for monomeric antibody clearance and suggest an additional function for circulating b cells. As a receptor for ige (fc r2), cd23 is a focus for ige immune complexes, leading to enhanced antigen presentation to t cells. as an adhesion molecule, cd23 interacts with cd21 to regulate ige production, germinal centre b cell survival and the presentation of soluble protein antigen by b cells to t cells. Transcript analysis of the in vitro derived b cell populations demonstrated expression profiles similar to maturing b cells in vivo. Loading with indo 1 was followed by staining for cd21 and cd23 to allow for gating for mz and fo b cells. representative data of nine experiments are shown. In this study, we show that mice with an inactivating mutation in the intramembrane protease signal peptide peptidase–like 2a (sppl2a) unexpectedly exhibit profound humoral immunodeficiency and lack mature b cell subsets, mirroring deficiency of the cytokine b cell–activating factor (baff).
Expression Of Igm Igd Cd23 And Cd27 Surface Molecules Identifying 4 As a receptor for ige (fc r2), cd23 is a focus for ige immune complexes, leading to enhanced antigen presentation to t cells. as an adhesion molecule, cd23 interacts with cd21 to regulate ige production, germinal centre b cell survival and the presentation of soluble protein antigen by b cells to t cells. Transcript analysis of the in vitro derived b cell populations demonstrated expression profiles similar to maturing b cells in vivo. Loading with indo 1 was followed by staining for cd21 and cd23 to allow for gating for mz and fo b cells. representative data of nine experiments are shown. In this study, we show that mice with an inactivating mutation in the intramembrane protease signal peptide peptidase–like 2a (sppl2a) unexpectedly exhibit profound humoral immunodeficiency and lack mature b cell subsets, mirroring deficiency of the cytokine b cell–activating factor (baff).
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